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onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="h78xn2j" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750107422/8042334.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="j3xikho" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津七色免疫組化染色服務(wù),免疫組化</p><p><span>***更新：</span>2020-08-11 01:11:09</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="l2ubyfw" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="ehovn7k" class="page-proshow02"><p class="title">詳細說明</p><div id="n33qszb" class="box"><p><p> <p> 為了在同一張組織切片或細胞學(xué)涂片上同時或先后顯示兩種或兩種以上的抗原，發(fā)展了雙重和多重免疫組化技術(shù)。這對于在原位了解不同抗原的關(guān)系，將形態(tài)與功能相結(jié)合極為有用。 </p> <p> 近年來，雙重標(biāo)記技術(shù)結(jié)合了免疫組化和核酸分子雜交技術(shù)，已跨越蛋白質(zhì)水平，進入DNA，天津七色免疫組化染色服務(wù)、RNA水平，天津七色免疫組化染色服務(wù)，可在同一細胞內(nèi)顯示兩種不同的DNA或mRNA，或者顯示DNA和蛋白質(zhì)。也有人以放射性同位素標(biāo)記和酶組織化學(xué)結(jié)合來顯示靶DNA和抗原，天津七色免疫組化染色服務(wù)。雙重染色的基本方法有雙重?zé)晒夂碗p重酶標(biāo)記法。雙重?zé)晒鈽?biāo)記**常用的是FITC和TMRITC或RB200，雙重酶標(biāo)記常用的是辣根過氧化物酶和堿性磷酸酶。 </p> 天津七色免疫組化染色服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津七色免疫組化染色服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750107422/8042334.png"></div><p style="text-indent:25px"> 近年來，隨著免疫組織化學(xué)技術(shù)的發(fā)展和各種特異性抗體的出現(xiàn)，使許多疑難**得到了明確診斷。在常規(guī)**病理診斷中，5%-10%的病例單靠H.E.染色難以作出明確的形態(tài)學(xué)診斷。尤其是免疫組化在**診斷和鑒別診斷中的實用價值受到了普遍的認可，其在低分化或未分化**的鑒別診斷時，準(zhǔn)確率可達50%-75%。免疫組織化學(xué)的臨床應(yīng)用主要包括以下幾方面：⑴惡性**的診斷與鑒別診斷；⑵確定轉(zhuǎn)移性惡性**的原發(fā)部位；⑶對某類**進行進一步的病理分型；⑷軟組織**的zhiliao一般需根據(jù)正確的組織學(xué)分類，因其種類多、組織形態(tài)相像，有時難以區(qū)分其組織來源，應(yīng)用多種標(biāo)志進行免疫組化研究對軟組織**的診斷是不可缺少的；⑸發(fā)現(xiàn)微小轉(zhuǎn)移灶，有助于臨床zhiliao方案的確定，包括手術(shù)范圍的確定。⑹為臨床提供zhiliao方案的選擇。天津多色熒光免疫組化</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津七色免疫組化染色服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748427735/7999973.png"></div><p> 抗體和抗原之間的結(jié)合具有高度的特異性，免疫組織化學(xué)正是利用了這一原理。先將組織或細胞中的某種化學(xué)物質(zhì)提取出來，以此作為抗原或半抗原，通過免疫動物后獲得特異性的抗體，再以此抗體去探測組織或細胞中的同類的抗原物質(zhì)。由于抗原與抗體的復(fù)合物是無色的，因此還必須借助于組織化學(xué)的方法將抗原抗體結(jié)合的部位顯示出來，以其達到對組織或細胞中的未知抗原進行定性，定位或定量的研究。免疫組織化學(xué)技術(shù)按照標(biāo)記物的種類可分為免疫熒光法、免疫酶法、免疫鐵蛋白法、免疫金法及放射免疫自顯影法等。這些常用免疫組織化學(xué)方法的原理如下：免疫熒光細胞化學(xué)技術(shù)將已知抗體標(biāo)上熒光素,以此作為探針檢查細胞或組織內(nèi)的相應(yīng)抗原,在熒光顯微鏡下觀察.當(dāng)抗原抗體復(fù)合物中的熒光素受激發(fā)光的照射后會發(fā)出一定波長的熒光。 </p><p> pH)漂洗5min×3/次，不時震蕩(洗去多余游離的熒光素標(biāo)記的抗體)?！ぞ彌_甘油封片–分析純無熒光的甘油9份+pH，。·鏡檢：在熒光顯微鏡下觀察。·優(yōu)點：方法簡便、特異性高，非特異性熒光染色少?！と秉c：敏感性偏低；而且每檢查一種抗原就需要制備一種熒光抗體。若檢測多種抗原需制備多種相應(yīng)的熒光標(biāo)記抗體。?直接免疫熒光法的注意事項·對熒光標(biāo)記的抗體的稀釋：要保證抗體的蛋白有一定的濃度；·一般稀釋度不應(yīng)超過1:20，抗體濃度過低，會導(dǎo)致產(chǎn)生的熒光過弱，影響結(jié)果的觀察。?直接免疫熒光法的注意事項(續(xù))·染色溫度和時間需要根據(jù)各種不同的標(biāo)本及抗原而變化；–染色時間：從10min到數(shù)小時，一般30min；–染色溫度：多采用室溫(25?C)，高于37?C可加強染色效果，但對不耐熱的抗原(如流行性乙型腦炎***)可采用0-2?C的低溫，延長染色時間。–低溫染色過夜較37?C30min效果好的多。?直接免疫熒光法的注意事項(續(xù))·試驗時需設(shè)置下列對照：–自發(fā)熒光對照(空白對照)：標(biāo)本加，。–陽性對照：用已知的陽性標(biāo)本加熒光標(biāo)記的特異性抗體。–特異性對照(***試驗)：標(biāo)本加未標(biāo)記的特異性抗體，再加熒光標(biāo)記的特異性抗體。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津七色免疫組化染色服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751093750/8037763.png"></div><p> 我單獨做了一次實驗來排除該因素。結(jié)果孵育2、5min時先出現(xiàn)背景，而特異性染色較淺，故這不符合常理，一般先出現(xiàn)特異性染色，然后隨著時間的延長，會出現(xiàn)非特異性背景著色。3、***，血清封閉的問題？以前我一般孵育15-30min，所以我單獨做了一次實驗用血清封閉室溫1h，其它條件同做出來的那一次，結(jié)果也一樣背景著色很深。4、此外，我在改進的同時，也對PBS清洗不斷地延長時間和增加次數(shù)，甚至完全參照試劑盒說明書來進行操作（我以前一般一抗4℃過夜且室溫復(fù)溫45min、二抗37℃30min、SP反應(yīng)37℃30min），以及過氧化氫滅活加強等等均未起到效果。我冷靜地分析了一下整個實驗流程，與***次做出來相比，只有兩個地方做了改動：因血清不夠而更換了不同廠家試劑盒、因抗體稀釋液用完而重新買了抗體稀釋液。5、我用PBS替代一抗稀釋液（我以前還回收抗體，自我覺得抗體稀釋液中含防腐劑和蛋白穩(wěn)定劑），其它步驟和組織切片完全與***次做出來的一致（包括血清也是老試劑盒內(nèi)剩余的一點），奇跡出現(xiàn)了：結(jié)果很好。因為抗原抗體反應(yīng)除溫度、抗原/抗體濃度外，然后就是pH值，于是我測了新抗體稀釋液、老抗體稀釋液和PBS的pH值，結(jié)果是前者偏酸性（<6、0），而后兩者均在7、5左右。天津多色熒光免疫組化</p><p style="text-indent:25px">天津七色免疫組化染色服務(wù)</p><p> 用甲醇配置過氧化氫比雙蒸水或PBS可能好在保護抗原和固定組織作用，過氧化氫孵育時間過長易引起脫片；現(xiàn)用現(xiàn)配，配好后4℃避光保存。不過，現(xiàn)在已有“第二代即用型免疫組化試劑盒”避免內(nèi)源性生物素的干擾，推薦使用。7）血清封閉組織切片上有剩余的位點可以與一抗非特異性結(jié)合，造成后續(xù)結(jié)果的假陽性；封閉血清一般是和二抗同一來源的，血清中動物自身的抗體，預(yù)先能和組織中有交叉反應(yīng)的位點發(fā)生結(jié)合；也可以用小牛血清、BSA、羊血清等，但不能與一抗來源一致。一般室溫10-30min。但也要防止封閉過度8）一抗和二抗?jié)舛群头跤龝r間一抗孵育條件在免疫組化反應(yīng)中**重要，包括孵育時間、溫度和抗體濃度。一抗孵育溫度有幾種：4℃、室溫、37℃，其中4℃效果比較好；孵育時間：這與溫度、抗體濃度有關(guān)，一般37℃1-2h，而4℃過夜和從冰箱拿出后37℃復(fù)溫45min。具體條件還要摸索。二抗孵育條件：二抗一般室溫或37℃30min-1h，具體時間需要摸索，而濃度一般有工作液，若是濃縮液還要摸索濃度。但在免疫組化中我們一般先把二抗?jié)舛群头跤龝r間先定下，然后去摸索一抗?jié)舛群头跤龝r間。建議一抗反應(yīng)在4℃比較好，反應(yīng)溫和，但時間比較好超過16~24h。天津七色免疫組化染色服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司始建于2003-01-22，坐落于上海市松江區(qū)廣富林路697號昂立大廈2113室，現(xiàn)有員工5~10人余人。zrbiorise,SPRICELL,scienceladde是上海朝瑞生物科技有限公司的主營品牌，是專業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)公司，擁有自己獨立的技術(shù)體系。公司以用心服務(wù)為核心價值,希望通過我們的專業(yè)水平和不懈努力,將1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)等業(yè)務(wù)進行到底。上海朝瑞科技始終以質(zhì)量為發(fā)展，把顧客的滿意作為公司發(fā)展的動力，致力于為顧客帶來高品質(zhì)的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]。</p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/875237.html</p></div></div></div></div></div><div id="vda39gq" class="page-right twoColRt"><div id="ti8i3ur" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="dov2o32" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/814588.html"><div><img 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