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src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289509208985/415071.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="82qrta9" class="content"><p><span>需求數量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產品關鍵詞：</span>藥用級泛解酸內酯生產</p><p><span>***更新：</span>2020-06-26 08:24:15</p><p><span id="click" >瀏覽次數：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="gh3i4co" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網址: </span></p><p><span>手機: </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="7za88xp" class="page-proshow02"><p class="title">詳細說明</p><div id="tipmjvp" class="box"><p><p style="text-indent:25px">其中Kataoka M 等發(fā)現(xiàn)來源于根*農桿菌的L-泛解酸內酯水解酶有較高的活力和選擇性。Kesseler M 等對根*農桿菌lu678 的L-泛解酸內酯水解酶構建大腸工程菌并過量表達該蛋白，酶活提高了200 倍，達到600 U/g。該過程要求L-泛解酸內酯必須完全水解，才能獲得高光學純度的D-泛解酸內酯，限制了其工業(yè)應用。D-泛解酸內酯水解酶選擇性水解拆分DL-泛解酸內酯生成D-泛解酸，藥用級泛解酸內酯生產，藥用級泛解酸內酯生產，再經內酯化生成D-泛解酸內酯，留下的L-泛解酸內酯經化學消旋化為DL-泛解酸內酯重新循環(huán)拆分。D-泛解酸內酯水解酶來源豐富，其中**常用的是鐮飽霉菌，有尖孢鐮刀菌、腐皮鐮刀菌和串珠鐮刀菌。2007 年，藥用級泛解酸內酯生產，SAKAMOTO K等利用固定化的尖孢鐮刀菌來水解拆分DL-泛解酸內酯，固定化細胞的半衰期可以達到6 000h，經過180次重復利用后仍然保持原活力的71%，相對于游離的整細胞催化其半衰期提高了35 倍。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="藥用級泛解酸內酯生產" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289509208985/415071.png"></div><p> 化學法與水解酶拆分法相結合合成D-泛解酸內酯憑借其成熟的工藝。目前在工業(yè)生產中占據***重要地位。隨著國內環(huán)保要求的日益趨嚴以及產能擴大帶來的競爭壓力，升級D-泛解酸內酯的合成工藝勢在必行。諸多的研究表明，微生物或者酶的應用有助于***、綠色地合成高光學純度的D-泛解酸內酯。內酯水解酶催化混旋泛解酸內酯的選擇性拆分已經成功地實現(xiàn)了工業(yè)生產。 </p> <p> 而羰基還原酶、醇脫氫酶和羥基腈裂合酶等也已在化學酶法合成D-泛解酸內酯方面展現(xiàn)出了巨 </p> <p> 大的應用潛力。后續(xù)利用基因工程、蛋白質工程以及反應工程等技術，提高酶的表達量，改善酶的催化性能，優(yōu)化催化反應條件，必將進一步提升化學酶法合成D-泛解酸內酯的技術競爭力。 </p> <p> <br /> </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="藥用級泛解酸內酯生產" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336133632812/8293857.png"></div><p> D-泛解酸內酯水解酶基因,基因片段長度為1146bp,該序列同來源于尖鐮孢霉菌(F.oxysporum)AKU 3702菌株的編碼D-泛解酸內酯水解酶cDNA結構基因的同源性為90.06%.將所得片段定向克隆到pTrc99a載體中,轉化至JM109感受態(tài)細胞,篩選出了陽性克隆.經IPTG誘導陽性菌,進行SDS-PAGE電泳,檢測出在約40kD處有一蛋白表達帶.對兩株重組基因工程菌的比活力進行測定,結果分別為37U和41U. </p> <p> <br /> </p> <p> 串珠鐮孢霉菌(Fusarium moniliforme)CGMCC 0536的D-泛解酸內酯水解酶具有高度的立體選擇性,可立體專一性拆分DL-泛解酸內酯,生成D-泛解酸. </p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/396733.html</p></div></div></div></div></div><div id="mwt2hq4" class="page-right twoColRt"><div id="zyfceqz" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="blcuwix" class="recomPic 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