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class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116738986650391/4176717.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="tskh3gy" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇四步9分鐘完成RNA提取試劑盒****</p><p><span>***更新：</span>2020-06-23 09:22:30</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="hr78xpr" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="u3dvfrj" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="oivxcoq" class="box"><p><p> 別名總RNA提取試劑盒英文名稱Total RNA Extraction Kit儲存條件2-8℃，江蘇四步9分鐘完成RNA提取試劑盒****，江蘇四步9分鐘完成RNA提取試劑盒****，避光，有效期1年單位盒 </p> <p> 貨號：R1200 </p> <p> 規(guī)格：50T/100T </p> <p> 產(chǎn)品內(nèi)容：試劑盒組成R1200-50R1200-100保存有效期裂解液50ml100ml2-8℃一年漂洗液15ml30mlRT一年洗柱液50ml100mlRT一年RNase free ddH2O15ml30mlRT一年RNase free吸附柱50個100個RT一年RNase free收集管(2ml)50個100個RT一年 </p> <p> <br /> </p> 操作步驟：樣品處理： a，江蘇四步9分鐘完成RNA提取試劑盒****. 植物組織：取新鮮或-70℃凍存100mg組織在液氮中研磨，把粉末加入到1ml裂解液中混勻。<div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四步9分鐘完成RNA提取試劑盒****" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116738986650391/4176717.png"></div><p> RNA 降解可能的原因如下： </p> <p> 組織取出后沒有馬上進(jìn)行處理或冷凍，導(dǎo)致 RNase 的釋放降解樣品中的RNA。 </p> <p> 貼壁細(xì)胞在胰蛋白酶處理時被破壞。 </p> <p> 樣本量太大而加入的 Trizol 液少，導(dǎo)致裂解不完全，不能有效*** RNase的活性，進(jìn)而導(dǎo)致 RNA 降解。 </p> <p> 提取過程中使用的溶液、離心管或***頭未經(jīng) RNase 去除處理。 </p> <p> RNA 沉淀沒有保存于-80°C。 </p> <p> RNA 電泳時的電泳液及電泳槽中含有 RNase，或者電泳時電泳液溫度過高，造成 RNA 樣品被降解。 </p> <p> 樣品中蛋白和多糖含量高，建議加入氯仿之前先高速離心去除蛋白和多糖。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四步9分鐘完成RNA提取試劑盒****" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414521485/5765636.png"></div><p> 用于各種植物、動物、微生物的RNA提取，在每個試劑盒里都有一份說明使用說明書。實驗者只需要按照說明書上的步驟操作即可。使用時無需配制其它試劑，非常方便，適合于RNA的快速提取。所提取的RNA完整性好、純度高，可用于分子生物學(xué)后實驗。但較好的試劑盒價格比較貴，在實驗室可以根據(jù)自己的實驗需要來定奪試劑盒的使用。 </p> <p> 一般包括：成分?jǐn)?shù)量儲藏溫度有效期裂解液100ml2-8℃一年漂洗液30mlRT一年洗柱液100mlRT一年RNasefreeddH2O30mlRT一年RNasefree吸附柱100個RT一年RNasefree收集管(2ml)100個RT一年此外，還配有一份試劑盒使用說明書，根據(jù)不同的生產(chǎn)商，可能還配有不同的試劑，如：DNA酶、溶菌酶等。 </p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/360354.html</p></div></div></div></div></div><div id="aumj3fc" class="page-right twoColRt"><div id="d2x7he7" class="box01 margin10 clearfix"><h3><a 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