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class="button">聯(lián)系我們</a></div></div><div id="7xeldk3" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海探普生物科技有限公司</a></p><p><span>聯(lián)系人：</span>喻利</p><p><span>郵箱: </span>380467011@qq.com</p><p><span>電話: </span>13166221237</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span>http://www.tpbio.com.cn</p><p><span>手機: </span>021-13166221</p><p><span>地址: </span>放鶴路1088號6號樓307</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="mgt8pea" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="zjbyqs3" class="box"><p><p style="text-indent:25px">深度測序與個性化醫(yī)學(xué)的范式相比，P4醫(yī)學(xué)更強調(diào)早期預(yù)測和預(yù)防，強調(diào)對患者了解的系統(tǒng)性和參與性。準(zhǔn)確醫(yī)學(xué)的概念則是在基因測序普及的基礎(chǔ)上，將整個個體的各種信息如生理信息（通過可穿戴設(shè)備可以即時監(jiān)控和收集到）和腸道菌群變化、各種組學(xué)信息（深度測序測定）整合，進(jìn)行準(zhǔn)確的疾病分型，國內(nèi)高通量測序分析多少錢、調(diào)整和預(yù)防。隨著老年化時代的到來及臨床資源的限制，國內(nèi)高通量測序分析多少錢，基于這三種范式的健康管理和準(zhǔn)確診療將成為生物醫(yī)學(xué)研究與應(yīng)用的基本范式，走向大眾生活，正如當(dāng)年的計算機發(fā)展歷程一樣，國內(nèi)高通量測序分析多少錢，會從原來的大型機器演變成可移動的小型工具。醫(yī)學(xué)與健康的將來也會隨著深度測序的普及和生物信息學(xué)數(shù)據(jù)處理能力的大幅度提高，而進(jìn)入個性化的大眾管理時代。DNA病毒基因組測序：獲得一種指定DNA病毒盡量完整的序列。國內(nèi)高通量測序分析多少錢</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="國內(nèi)高通量測序分析多少錢,病毒全基因組測序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444035161598648/3163157.png"></div><p style="text-indent:25px">近年來，第二代測序技術(shù)(next gene -ration sequencing, NGS)發(fā)展迅猛，與 Sanger 測序技術(shù)相比, NGS 是一種能一次對幾十萬到幾百萬的DNA 分子進(jìn)行序列測定的高通量的測序技術(shù), 這種高通量測序使得對一個物種的轉(zhuǎn)錄組和基因組進(jìn)行細(xì)致全貌地分析成為可能, 因此又被稱為深度測序(deep sequencing). 相比傳統(tǒng)的個體基因組測序, NGS 使得測序價格日益廉價, 并且在生物信息學(xué)軟件的輔助下, 可以將大量不同基因片段的信息連接起來進(jìn)行基因組組裝, 完成生物的基因組測序，這種新的測序技術(shù)革新了植物病毒的診斷方法, 對于病毒的流行病學(xué)和生態(tài)學(xué)研究起到了非常重要的推動作用。國內(nèi)高通量測序分析多少錢病毒全基因組測序產(chǎn)品特點：不預(yù)設(shè)目標(biāo)檢出物，樣本的所有病原信息一網(wǎng)打盡。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="國內(nèi)高通量測序分析多少錢,病毒全基因組測序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444035048394729/6383653.png"></div><p style="text-indent:25px">對病毒的全基因組進(jìn)行測序是通過非特異性擴增+克隆結(jié)合sanger測序來完成的。當(dāng)物種有了參考的序列之后，可以通過特異性擴增+sanger測序獲得全基因組序列。Sanger測序準(zhǔn)確度高，讀長很長，但與此同時，擴增和克隆工作費時費力，由于流程繁瑣，加上快速變異導(dǎo)致引物無法通用，該方法對于大量基因組的測序工作而言，可操作性不強，這對于研究者一直是一個困擾。高通量測序技術(shù)正式啟用之后，研究者可以將樣品處理至標(biāo)準(zhǔn)濃度和體積后進(jìn)行測序和分析，減少了工作量，增加了成功率。探普生物進(jìn)行了大量有針對性的研發(fā)和測試，開發(fā)了全套的實驗和分析流程用于對病毒的全基因組進(jìn)行測序，該流程自運行以來廣受研究者們好評。</p><p style="text-indent:25px">哪些應(yīng)用場景需要對病毒的全基因組進(jìn)行測序？在探普生物長時間運行過程中，我們接觸到的對病毒的全基因組進(jìn)行測序項目有比較豐富的應(yīng)用場景。先，從事基因進(jìn)化/疫苗/藥品/抗體研制方向的研究的研究者一定會用到測序。這種場景一般是用密集的sanger測序監(jiān)測某幾個關(guān)鍵基因，搭載一定頻率的全基因組測序。這樣的組合省時省力省經(jīng)費，同時能達(dá)到研究目的。此外，有的單位需要對傳染病的病原進(jìn)行流行病學(xué)監(jiān)測和研究，如疾控/疫控中心、醫(yī)院的傳染病科室以及一些高校和研究所的相應(yīng)課題組，可能需要對病毒的全基因組進(jìn)行測序以后，結(jié)合其他上下游的研究數(shù)據(jù)，達(dá)到研究或者監(jiān)測疫病的目的。上海探普生物科技有限公司的對病毒的全基因組進(jìn)行測序有什么優(yōu)勢？</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="國內(nèi)高通量測序分析多少錢,病毒全基因組測序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444034988902408/4936035.png"></div><p style="text-indent:25px">利用病毒傳播過程中核酸序列上特定位置的變化來進(jìn)行分型，著重于區(qū)分不同型別病毒的來源，是我國調(diào)整防控策略的重要依據(jù)之一。傳統(tǒng)的病原微生物檢測手段包括形態(tài)學(xué)檢測、培養(yǎng)分離、生化檢測和免疫學(xué)檢測。這些方法檢測周期長、靈敏度低，對操作人員的技術(shù)水平要求比較高等因素；熒光定量PCR技術(shù)和等溫擴增技術(shù)等分子生物學(xué)的檢測方法部分解決了上述問題，簡單快速，通過對核酸特異性序列的檢測，可在短時間內(nèi)快速判斷病原體的種類，但是這些方法無法進(jìn)行混合傳染鑒定和病毒溯源，隨著基因組學(xué)技術(shù)的發(fā)展，高通量測序技術(shù)已經(jīng)能夠做到不依賴于傳統(tǒng)的微生物培養(yǎng)，可直接對臨床樣本中的核酸進(jìn)行高通量測序，然后與數(shù)據(jù)庫進(jìn)行比對，實現(xiàn)傳染性疾病的溯源、檢測、分型和耐藥評估等多個方面，受到越來越多臨床和科研工作者的關(guān)注。高通量測序技術(shù)正式啟用之后，研究者可以將樣品處理至標(biāo)準(zhǔn)濃度和體積后進(jìn)行測序和分析，減少了工作量。國內(nèi)高通量測序分析多少錢</p><p style="text-indent:25px">在探普生物長時間運行過程中，我們接觸到的對病毒的全基因組進(jìn)行測序項目有比較豐富的應(yīng)用場景。國內(nèi)高通量測序分析多少錢</p><p style="text-indent:25px">全基因組測序是對未知基因組序列的物種進(jìn)行個體的基因組測序。技術(shù)路線：提取基因組DNA，然后隨機打斷，電泳回收所需長度的DNA的片段（0.2~5Kb），加上接頭, 進(jìn)行DNA簇（Cluster）制備，后利用Paired-End（Solexa）或者M(jìn)ate-Pair（SOLiD）的方法對插入片段進(jìn)行測序。然后對測得的序列組裝成Contig，通過Paired-End的距離可進(jìn)一步組裝成Scaffold，進(jìn)而可組裝成染色體等。組裝效果與測序深度與覆蓋度、測序質(zhì)量等有關(guān)。常用的組裝有：SOAPdenovo、Trimity、Abyss等。測序深度（Sequencing depth）是指測序得到的堿基總量（bp）與基因組大小的比值，它是評價測序量的指標(biāo)之一。測序深度與基因組覆蓋度之間是一個正相關(guān)的關(guān)系，測序帶來的錯誤率或假陽性結(jié)果會隨著測序深度的提升而下降。測序的個體，如果采用的是雙末端或Mate-Pair方案，當(dāng)測序深度在50X~100X以上時，基因組覆蓋度和測序錯誤率控制均得以保證，后續(xù)序列組裝成染色體才能變得更容易與準(zhǔn)確。國內(nèi)高通量測序分析多少錢</p><p style="text-indent:25px">上海探普生物科技有限公司致力于醫(yī)藥健康，是一家服務(wù)型的公司。上海探普生物致力于為客戶提供良好的病毒測序，病毒全基因組測序，病毒宏基因組測序，未知病原鑒定，一切以用戶需求為中心，深受廣大客戶的歡迎。公司秉持誠信為本的經(jīng)營理念，在醫(yī)藥健康深耕多年，以技術(shù)為先導(dǎo)，以自主產(chǎn)品為重點，發(fā)揮人才優(yōu)勢，打造醫(yī)藥健康良好品牌。上海探普生物秉承“客戶為尊、服務(wù)為榮、創(chuàng)意為先、技術(shù)為實”的經(jīng)營理念，全力打造公司的重點競爭力。</p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/3149313.html</p></div></div></div></div></div><div id="339o8zb" class="page-right twoColRt"><div id="ixphj2o" class="box01 margin10 clearfix"><h3><a 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