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</a></p></div></div><div id="78vp7lx" class="page-proshow02"><p class="title">詳細說明</p><div id="ico2yv3" class="box"><p><p> 蜂毒肽發(fā)揮其眾多功能的主要機理是因為蜂毒肽可以使細腦膜的透性增加，細胞內(nèi)容物泄露，細胞裂解所以蜂毒肽與天然或是模型膜的作用成為蜂毒肽研究的熱點。Benachir等利用鈣黃綠素作為熒光標記物，系統(tǒng)研究了蜂毒肽誘導的膜滲漏。他們認為蜂毒肽與囊泡的結(jié)合會非常迅速，因為在蜂毒肽作用幾分鐘之后，內(nèi)容物的流出達.到**大值，并且停止;而且蜂毒肽誘導的磷脂雙分子層的裂解的發(fā)生 </p> <p> 機制是“全或無”，也就是說蜂毒肽作用下，北京蜂毒，有的囊泡的內(nèi)容物全部釋放，而有的則保持完整，北京蜂毒，沒有滲漏，北京蜂毒，這與Schwarz等觀察的一致。蜂毒含有的多肽類、酶類及胺類等成分能刺激肌膚產(chǎn)生天然的膠原蛋白。北京蜂毒</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京蜂毒,蜂毒" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637293699074541015/5782620.png"></div><p> 容物釋放的比例與蜂毒肽/脂質(zhì)的摩爾比率有關(guān)。更為特別的是蜂毒肽可以區(qū)別出完整的和已經(jīng)有物質(zhì)滲出的囊泡，而且表明卵磷脂雙分子層表面負電荷的存在對蜂毒肽的裂解能力有***作用，并且與負電荷的密度成一定比例。 </p> <p> <br /> </p> <p> 1999年，Kubo等通過5種細胞毒的測定方法對蜂毒肽與嗜酸性粒細胞的主要堿性蛋白進行比較，結(jié)果證實蜂毒肽能插入K562細胞的胞膜中進而形成孔道，引起Ca內(nèi)流，使胞內(nèi)Ca濃度升高，細胞裂解。1小時內(nèi)，蜂毒肽對實驗的白血病細胞均具殺傷效果。Shamsher等研究發(fā)現(xiàn)蜂毒肽可***磷酸脂酶D進而裂解人單核白血病細胞(U937)。北京蜂毒蜂毒貯存在毒囊中的墊刺里，由蟄針排出。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京蜂毒,蜂毒" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637291456095595704/9364780.png"></div><p> 蜂毒肽可以作用多種酶及細胞蛋白，在裂解的細胞中蜂毒肽能結(jié)合或改變幾種蛋白質(zhì)功能。如蜂毒肽可以***蛋白激酶C;與鈣調(diào)蛋白(calmodulin)具有高度親極性。Fukushi-ma 實驗中觀察到蜂毒肽***小鼠大腦皮質(zhì)的突觸膜_上依賴鳥苷酸的腺苷酸環(huán)化酶活性;還發(fā)現(xiàn)蜂毒肽能明顯刺激Gi1和G11活性，但卻***Gs活性。動力學研究表明蜂毒肽***Gs活性是因為***GDP的釋放，從而增加了鳥苷酸造成的。而***Gi與***Gs很可能與***腺苷酸環(huán)化酶的活性有關(guān)。細胞脂酶的活性產(chǎn)物包括三磷酸肌醇(IP3)、甘油二酯(DG)和脂肪酸(FA)等是細胞生理活動重要的第二信使系統(tǒng)，而有實驗表明蜂毒.肽可以***細胞脂酶，包括磷脂酶C(PLC)、磷脂酶D(PLD)、磷脂酶Az(PLAJ及甘油三酯酶等。磷脂和甘油三酯是細胞內(nèi)主要的?；?包含脂肪酸)。而甘油三酯的相對含量可占細胞總脂量的5% ~ 60%。 </p> <p> <br /> </p><p> 蜂毒(venom)存在于蜜蜂毒囊內(nèi)的毒液，古碳，氫、硫磷、鈣、氯、氮等元素，系透明液體，呈酸性反應。蜂毒是具有高度藥理學和生物學活性的復雜混和物，主要為蜂毒肽括性酶生物胺、蜂毒肥大細胞脫粒肽等lO多種活性肽=此外，還有透明質(zhì)酸酶、蜂毒磷脂酶及組胺等多種活性物質(zhì)，故有肽類“寶庫”之稱。 </p> <p style="text-align:left;"> <p> 蜂毒肽(mPlilfin)是蜂毒的主要成分．約占蜂毒于重的50％，是蜂毒中起藥理作用和生物學活性的主要組分蜂毒肽由26個氨基酸組成，是不對稱的線性多肽，第1位至第 </p> <p> 10位的氨基酸是疏水基團，第2l至第26位的氨基酸是親水基團由于蜂毒肽具有結(jié)構(gòu)已知及膜活性的特點，現(xiàn)已成為研究細胞作用機制，鈣調(diào)蛋白作用機制的常用探針。 </p> <p> <br /> </p> 在室溫下能很快干燥成白色或淡黃色晶體狀。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京蜂毒,蜂毒" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637291455686855469/573768.png"></div><p> 蜂毒肽的螺旋結(jié)構(gòu)(二、三級結(jié)構(gòu))和聚合作用取決于溶液的PH值、離子強度和蜂毒肽本身的濃度。當濃度的PH值升高后，蜂毒肽能從大部分隨機構(gòu)象轉(zhuǎn)變成。螺旋構(gòu)象，0螺旋構(gòu)象含量比較高可達80%。當溶液PH處于個性狀態(tài)時?蜂毒肽至少有三種構(gòu)象存在。蜂毒肽的發(fā)現(xiàn)及純化程度的進一步提高以及人工合成的成功，證明了一類新的有生物活性的肽類結(jié)構(gòu)，為研究多肽與脂質(zhì)膠?；蚣毎ぶg相互作用提供了有效的方法;同時對蜂毒肽的多種生物活性和多種藥理作用提供了相關(guān)機理的解釋。 </p> <p> <br /> </p> <p> 關(guān)于蜂毒肽抑瘤作用的量效關(guān)系，與蜂毒肽本身的理化性質(zhì)和在高濃度溶液的自聚有關(guān)。在低濃度時，蜂毒肽以單體狀態(tài)存在，不利于膜上離子通道的形成，雖能插膜，但對**細胞的破壞較弱;在高濃度時，蜂毒肽多形成四聚體狀態(tài)，能比單體更有效地與細胞膜結(jié)合,形成離子通道，改變了膜的通透性，造成細胞膜的破裂，從而顯示出***的體外殺傷**的作用。由于蜂毒來源豐富．作用廣，功效大，因此蜂毒的藥理作用和***價值將會有一個廣悶發(fā)展前景。北京蜂毒</p><p style="text-indent:25px">蜂毒是蜜蜂和馬蜂等蜂類毒腺分泌的0液體。北京蜂毒</p><p style="text-indent:25px">內(nèi)容物釋放的比例與蜂毒肽/脂質(zhì)的摩爾比率有關(guān)，只有達到一定量的蜂毒肽才可能有內(nèi)容物滲出。更為特別的是蜂毒肽可以區(qū)別出完整的和已經(jīng)有物質(zhì)滲出的囊泡，說明這兩類脂質(zhì)雙分子層的性質(zhì)有很大.的不同。**近有很多報道認為，微粒表面的負電荷可以***蜂毒肽對細胞的裂解作用，Benachir 等的工作表明卵磷脂雙分子層表面負電荷的存在對蜂毒肽的裂解能力有***作用，并且與負電荷的密度成一定比例。這可能是由于靜電作用吸附蜂毒肽，而不能更加深入破壞囊.泡而形成滲漏。北京蜂毒</p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/3011964.html</p></div></div></div></div></div><div id="2ofhwxw" class="page-right twoColRt"><div id="rbdkmtv" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="nxzmta3" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/814588.html"><div><img 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