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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇四色免疫組化檢測服務(wù),免疫組化</p><p><span>***更新：</span>2020-06-13 17:13:32</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="8as2x4t" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="2g2byvc" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="3eya3gi" class="box"><p><p> 具體時(shí)間需要摸索，而濃度一般有工作液，若是濃縮液還要摸索濃度，切記要避光反應(yīng)。但在免疫熒光中我們一般先把二抗?jié)舛群头跤龝r(shí)間先定下，然后去摸索一抗?jié)舛群头跤龝r(shí)間。***，熒光素標(biāo)記的二抗隨著保存時(shí)間的延長，可能后有大量的游離熒光素殘留，需要注意配制時(shí)小包裝和并進(jìn)行適當(dāng)?shù)碾x心。6）復(fù)染目的是形成細(xì)胞輪廓，從而更好對目標(biāo)蛋白進(jìn)行定位。一般常用DAPI復(fù)染。7）封片為了長期保存，我們一般用緩沖甘油等封片，此外還有專門的抗熒光萃滅封片液。避免產(chǎn)生氣泡，方法是直接在載玻片組織上滴一滴封片液，然后一手拿住蓋片某一拐角，而另一手拿對面的那個(gè)拐角，接近封片液近端的拐角先降低，直至接觸到液體時(shí)為止；當(dāng)發(fā)現(xiàn)液體接觸面在不斷彌散時(shí)，則可以緩慢降低另一拐角，這樣一般不會產(chǎn)生氣泡。8）切片清洗為了防止一抗、二抗等試劑殘留而引起非特異性染色，所以適當(dāng)?shù)丶訌?qiáng)清洗（延長時(shí)間和增多次數(shù)）尤為重要，我一般在一抗孵育前的清洗是3min*3次，江蘇四色免疫組化檢測服務(wù)，而一抗孵育后的清洗均為5次*5min。注意：⑴單獨(dú)沖洗，防止交叉反應(yīng)造成污染，江蘇四色免疫組化檢測服務(wù)。⑵溫柔沖洗，防止切片的脫落，江蘇四色免疫組化檢測服務(wù)。我喜歡用浸洗方式；⑶沖洗的時(shí)間要足夠，才能徹底洗去結(jié)合的物質(zhì)。⑷PBS的PH和離子強(qiáng)度的使用和要求。江蘇四色免疫組化檢測服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四色免疫組化檢測服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750458985/5221034.png"></div><p> 使用的玻片等載體，都必須厚度均勻，無明顯的自發(fā)熒光，如果使用油鏡，還必須保證鏡油為無熒光鏡油；電源比較好裝穩(wěn)壓器，否則電壓不穩(wěn)不僅會降低汞燈的壽命，也會影響鏡檢的效果。3、免疫組化和免疫熒光結(jié)果分析：見***場試驗(yàn)講座。案例一DAB染色后切片著色一片黃/背景深背景：奧運(yùn)會期間我們課題組研究生都在實(shí)驗(yàn)室做實(shí)驗(yàn)，許多從北京購買的試劑買不到，對我們的許多實(shí)驗(yàn)產(chǎn)生了很大的影響。我以前一直用中杉金橋的SP三步法染色試劑盒，效果不錯(cuò)，在奧運(yùn)會期間我準(zhǔn)備做同批實(shí)驗(yàn)分不同批次酶免疫組化實(shí)驗(yàn)，***批組化實(shí)驗(yàn)結(jié)果很好，抗體濃度感覺比較合適（SantaCruz公司1：200），等做第二批時(shí)發(fā)現(xiàn)封閉血清不夠了，為了保證實(shí)驗(yàn)順利進(jìn)行，我又從福州邁新頂了一個(gè)SP試劑盒，同時(shí)抗體稀釋液也不夠了，我又重新從博士德公司買了一小瓶10ml。從第二批實(shí)驗(yàn)開始，組化實(shí)驗(yàn)結(jié)果一直顯示強(qiáng)背景著色，特異性染色很難辨別。后來我將標(biāo)本拿到上海舜田生物去做，做了效果很不錯(cuò)，于是我就請教了他們公司從事病理30多年經(jīng)驗(yàn)的老師，她告訴我應(yīng)該如何去分析和解決問題，很快我就找到了思路，結(jié)果問題終于解決了。天津六色免疫組化染色服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四色免疫組化檢測服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899754550782/2606693.png"></div><p> 因而它是大多數(shù)免疫組化中優(yōu)先的組織標(biāo)本制作方法。1、取材的特殊要求及注意事項(xiàng)·標(biāo)本新鮮：一般在2h以內(nèi)進(jìn)行，超過2h，組織將有不同程度的自溶，其抗原或變性消失，或嚴(yán)重彌散?！と〔牟课唬撼〔≡罨蚝龣z抗原部位外，還應(yīng)取病灶與正常交界處，即所取組織切片中同時(shí)應(yīng)有抗原陽性和陰性區(qū)，以形成自身對照。–細(xì)胞壞死后，不僅抗原彌散或消失，而且常引起非特異著色，干擾觀察，因此取材時(shí)應(yīng)盡可能避開壞死區(qū)。1、取材的特殊要求及注意事項(xiàng)(續(xù))·避免擠壓：取材時(shí)組織受擠壓可使邊緣部細(xì)胞形態(tài)改變并加深非特異著色，因而取材時(shí)應(yīng)使用鋒利的刀刃；–鑷取組織動作要輕；–經(jīng)窺鏡直接鉗取的組織往往有過度擠壓，觀察結(jié)果時(shí)應(yīng)有所考慮。2、固定及常用的固定液·取材后的組織需立刻投于固定劑中–固定使組織和細(xì)胞的蛋白質(zhì)凝固，終止內(nèi)源性或外源性酶反應(yīng)，防止組織自溶或異溶，以保持原有結(jié)構(gòu)和形態(tài)；–對免疫組化而言更有原位保存抗原的作用，避免抗原失活或彌散?！こＳ霉潭ㄒ海汗潭▌┢贩N很多，但大多屬于醛類和醇類。其固定原理不同，各有優(yōu)缺點(diǎn)。–醛類（常用甲醛、戊二醛和多聚甲醛）–醇類（常用乙醇）–其它（**）(1)醛類·甲醛。</p><p style="text-indent:25px">【多色免疫組化染色樣本要求】1福爾馬林固定的蠟塊或玻片，大塊組織或TMA都可以，封蠟不能有明顯的破損。2玻片樣本，組織需要緊貼玻片，避免褶皺，玻片不能有破損、劃傷或污漬。3組織**小應(yīng)包含大于1000個(gè)細(xì)胞。4蠟塊需包埋實(shí)體瘤組織，壞死瘤組織、細(xì)針穿刺物、細(xì)胞甩片樣品會影響染色效果。5組織應(yīng)當(dāng)用10%中性福爾馬林固定，正常固定時(shí)間為18～24小時(shí)。6切片厚度為4μm左右，使用防脫玻片。建議玻片在固定后一周內(nèi)制備為佳。7不要在水浴撈片環(huán)節(jié)添加任何粘合劑，樣本應(yīng)置于玻片正面、**。將撈片豎直置于吸水紙上去除水分，輕敲去除水滴，不要用紙擦拭玻片。8玻片置于45°C熱盤上放置30min（自然風(fēng)干玻片時(shí)長不小于1小時(shí)）。【設(shè)備耗材】1檢驗(yàn)所需設(shè)備器材：熒光顯微鏡、通風(fēng)櫥、移液器、恒溫干燥箱、微波爐、計(jì)時(shí)器2檢測所需耗材器皿：免疫組化筆、微波修復(fù)杯、染色缸、孵育濕盒、蓋玻片、洗瓶、量筒100ml、量筒1000ml等【試劑制備】1通用試劑：DMSO、miejun去離子水、二甲苯、乙醇（全部、95%、70%）、10%中性福爾馬林、抗原修復(fù)液（推薦使用佰諾全景酸性或堿性抗原修復(fù)液）、一抗封閉液等。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四色免疫組化檢測服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899749443360/7546740.png"></div><p> 收藏查看我的收藏0有用+1已投票0免疫組化編輯鎖定討論免疫組化，是應(yīng)用免疫學(xué)基本原理——抗原抗體反應(yīng)，即抗原與抗體特異性結(jié)合的原理，通過化學(xué)反應(yīng)使標(biāo)記抗體的顯色劑（熒光素、酶、金屬離子、同位素）顯色來確定組織細(xì)胞內(nèi)抗原（多肽和蛋白質(zhì)），對其進(jìn)行定位、定性及相對定量的研究，稱為免疫組織化學(xué)技術(shù)(immunohistochemistry)或免疫細(xì)胞化學(xué)技術(shù)(immunocytochemistry)。中文名免疫組化外文名immunocytochemistry應(yīng)用免疫學(xué)基本原理稱為免疫組織化學(xué)技術(shù)目錄1基本原理2分類3作用?標(biāo)本?抗體?常用染色方法4操作步驟?操作步驟?化染色步驟5判定分析6意義?免疫組化鏡檢?細(xì)胞凋亡檢測?關(guān)于掉片7經(jīng)驗(yàn)總結(jié)?實(shí)驗(yàn)為例?免疫個(gè)人感悟免疫組化基本原理編輯抗體和抗原之間的結(jié)合具有高度的特異性，免疫組織化學(xué)正是利用了這一原理。先將組織或細(xì)胞中的某種化學(xué)物質(zhì)提取出來，以此作為抗原或半抗原，通過免疫動物后獲得特異性的抗體，再以此抗體去探測組織或細(xì)胞中的同類的抗原物質(zhì)。由于抗原與抗體的復(fù)合物是無色的，因此還必須借助于組織化學(xué)的方法將抗原抗體結(jié)合的部位顯示出來，以其達(dá)到對組織或細(xì)胞中的未知抗原進(jìn)行定性，定位或定量的研究。江蘇四色免疫組化檢測服務(wù)</p><p style="text-indent:25px">江蘇四色免疫組化檢測服務(wù)</p><p> 問題及其解答：產(chǎn)生組織切片非特異性染色的原因有哪些？如何解決？1、抗體孵育時(shí)間過長、抗體濃度高易增加背景著色。這可通過縮短一抗/二抗孵育時(shí)間、稀釋抗體來控制。這是**重要的一條。2、一抗用多克隆抗體易出現(xiàn)非特異性著色，建議試用單克隆抗體看看。3、內(nèi)源性過氧化物酶和生物素在肝臟、腎臟等組織含量很高（含血細(xì)胞多的組織），需要通過延長滅活時(shí)間和增加滅活劑濃度來降低背景染色；4、非特異性組分與抗體結(jié)合，這需要通過延長二抗來源的動物免疫血清封閉時(shí)間和適當(dāng)增加濃度來加強(qiáng)封閉效果；5、DAB孵育時(shí)間過長或濃度過高；6、PBS沖洗不充分，殘留抗體結(jié)果增強(qiáng)著色，在一抗/二抗/SP孵育后的浸洗尤為重要；7、標(biāo)本染色過程中經(jīng)常出現(xiàn)干片，這容易增強(qiáng)非特異性著色。我的實(shí)際解決方案：以上的分析可能對于初學(xué)者還是不容易的，下面我就把我的排除實(shí)驗(yàn)的具體過程與大家進(jìn)行分享、交流和討論。1、首先排除抗體孵育條件。一般來說，著色效果的好壞與抗體的孵育條件是密不可分的，由于用SP法已經(jīng)做出來過一次且效果不錯(cuò)，因此對于同樣組織的同一抗原進(jìn)行分析，這種因素可以先排除。2、其次，DAB孵育時(shí)間是否太長？做出來那一次我是孵育8min，后來也是8-10min。江蘇四色免疫組化檢測服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司坐落在上海市松江區(qū)廣富林路697號昂立大廈2113室，是一家專業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)公司。目前我公司在職員工達(dá)到5~10人人，是一個(gè)有活力有能力有創(chuàng)新精神的高效團(tuán)隊(duì)。公司以誠信為本，業(yè)務(wù)領(lǐng)域涵蓋[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]，我們本著對客戶負(fù)責(zé)，對員工負(fù)責(zé)，更是對公司發(fā)展負(fù)責(zé)的態(tài)度，爭取做到讓每位客戶滿意。公司憑著雄厚的技術(shù)力量、飽滿的工作態(tài)度、扎實(shí)的工作作風(fēng)、良好的職業(yè)道德，樹立了優(yōu)良的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]形象，贏得了社會各界的信任和認(rèn)可。</p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/248115.html</p></div></div></div></div></div><div id="zdanpwy" class="page-right twoColRt"><div id="vkmjby7" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="8yvcja3" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" 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