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01:07:34</p><p><span id="click" >瀏覽次數(shù)：1次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="jynegx3" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">昆山伯利克精密儀器有限公司</a></p><p><span>聯(lián)系人：</span>唐經(jīng)理</p><p><span>郵箱: </span>sales@biolytic.cn</p><p><span>電話: </span>18051907886</p><p><span>傳真: </span>0512_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>0512-55270018</p><p><span>地址: </span>玉楊路1038號1號樓201室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="kjdkmov" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="v877zgi" class="box"><p><p> DNA合成儀設(shè)計(jì)用于合成結(jié)構(gòu)上類似DNA或RNA的寡核苷酸的自動化儀器。一般應(yīng)用于固相合成法，每次將一個核苷酸加到所接長的寡核苷酸鏈上，加入每一個核苷酸都利用相同的化學(xué)反應(yīng)與相應(yīng)的嘌呤或嘧啶堿基衍生物作用，天津進(jìn)口基因合成儀銷售。所用化學(xué)反應(yīng)和操作細(xì)節(jié)隨不同的儀器而改變，**廣泛應(yīng)用的方法是DNA合成的磷酸酰胺法。中文名DNA合成儀目錄1結(jié)構(gòu)和性能2操作步驟3日常維護(hù)4特點(diǎn)與性能5發(fā)展DNA合成儀結(jié)構(gòu)和性能編輯試劑驅(qū)動系統(tǒng)一般地，DNA合成儀采用一定壓力的惰性氣體（氬氣）或氮?dú)饧半姶砰y組驅(qū)動液體試劑，也可采用氦氣驅(qū)動試劑。某些合成儀采用sliderblock滑塊系統(tǒng)及精密蠕動泵，可不采用氣體為驅(qū)動系統(tǒng)。采用氣體及電磁閥驅(qū)動液體試劑時，需首先將管路系統(tǒng)電磁閥前段，含試劑瓶組中壓力加壓到規(guī)定氣壓，通過合成管理軟件或手動打開電磁閥（一般打開時間為數(shù)ms），即可驅(qū)動液體試劑到所需的合成柱中。試劑和堿基瓶組DNA合成儀一般需堿基瓶組及試劑瓶組。試劑瓶組一般**少為6個，含脫保護(hù)劑（Deb）、活化劑（act）、蓋帽試劑A（capA)、蓋帽試劑B(capB)、氧化劑（OXI）及洗脫乙腈（），每種儀器一般都多設(shè)置1-2個試劑瓶位，用于特殊產(chǎn)物的合成，天津進(jìn)口基因合成儀銷售。堿基瓶組一般**少為4個標(biāo)準(zhǔn)堿基（A/C/G/T），天津進(jìn)口基因合成儀銷售。試劑通過底部的luer接頭流到柱子，如果沒擰上下面的一個luer接頭應(yīng)會在一個圓柱形的玻璃流路節(jié)流閥上發(fā)現(xiàn)。天津進(jìn)口基因合成儀銷售</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津進(jìn)口基因合成儀銷售,基因合成儀" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364679619141/6467420.jpg"></div><p> 發(fā)展：編輯當(dāng)前DNA合成儀的主要生產(chǎn)廠商都致力于機(jī)器性能的完善和應(yīng)用領(lǐng)域的開拓以及高效率、高產(chǎn)率的儀器的開發(fā)與研究。中國臺灣科學(xué)委yuan會“基因醫(yī)藥衛(wèi)生科技尖duan研究計(jì)劃”中的基因生物技術(shù)組已經(jīng)成功研發(fā)全世界第yi臺高密度復(fù)制DNA合成儀，可以同時合成384條不同DNA，每日可合成768條DNA，并可以配合聚合酶連鎖反應(yīng)裝置，大量復(fù)制各種基因，不但節(jié)省時間，也可節(jié)省大量人力，這部機(jī)器能復(fù)制動物、人體、植物的基因甚至是防偽基因。由于人們所需要的大部分核酸的堿基對數(shù)量遠(yuǎn)遠(yuǎn)超過DNA合成儀可以合成的**長核酸鏈的堿基對數(shù)量，因此還需要不斷改善合成工藝才能得到較長的核酸分子。為了能夠在將來改變酶的結(jié)構(gòu)，使其在工業(yè)上更有價值；改變蛋白質(zhì)和多肽的結(jié)構(gòu)，制備新藥wu；并開拓有關(guān)人類疾病和遺傳調(diào)控的新領(lǐng)域，化學(xué)合成DNA在這一過程中將起關(guān)鍵性作用。根據(jù)不同化學(xué)方法研制的DNA合成儀也將不斷涌現(xiàn)，能夠突破現(xiàn)有核酸合成的堿基對數(shù)量限制，大量節(jié)省人力、物力、財力。浙江口碑好基因合成儀銷售電話將標(biāo)記好的清潔的收集瓶裝在合成儀上。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津進(jìn)口基因合成儀銷售,基因合成儀" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364679628907/6766793.jpg"></div><p> 【基因合成流程】 </p> <p> 1. 通過使用計(jì)算機(jī)軟件設(shè)計(jì)DNA序列，這些DNA序列可構(gòu)成生物電路或通路(圖1)。 </p> <p> 2. DNA序列被分割成更小的重疊片段(通常為200-1500bp的片段)，然后進(jìn)一步分割成可化學(xué)合成的一組單鏈寡核苷酸。 </p> <p> 3. 單鏈寡核苷酸通過各種DNA組裝技術(shù)，**終被組裝成更大的DNA片段并進(jìn)行克隆驗(yàn)證。 </p> <p> 4. 驗(yàn)證后的DNA序列被轉(zhuǎn)入細(xì)胞進(jìn)行功能測試。 </p> <p> 5. 根據(jù)功能試驗(yàn)結(jié)果，對本輪設(shè)計(jì)序列進(jìn)行更改，并重復(fù)測試周期，形成迭代。 </p> <p> 圖1：基因合成的循環(huán)過程。（來源：Cold Spring Harb Perspect Biol） </p> <p> <br /> </p><p> 儀器簡介：XH-400UV紫外光催化平行合成儀是利用紫外光的光化學(xué)原理，使反應(yīng)物分子吸收光子激發(fā)電子，分子由基態(tài)提升到激發(fā)態(tài)，從而發(fā)生光化學(xué)反應(yīng)。該儀器結(jié)合現(xiàn)代智能控制技術(shù)，在提高化學(xué)合成速度，有機(jī)物降解、工業(yè)廢水處理和環(huán)境污染等方面的研究提供了良好的方法和實(shí)驗(yàn)數(shù)據(jù)。技術(shù)參數(shù)：1．紫外汞燈光源功率：400W；100-400W功率可調(diào)；2．紫外光波長：365nm3．接觸式耐腐蝕溫度傳感器，實(shí)時監(jiān)測反應(yīng)溫度；4．轉(zhuǎn)盤轉(zhuǎn)速：10轉(zhuǎn)/分；5．?dāng)?shù)碼管顯示：時間、當(dāng)前溫度；6．反應(yīng)容器：石英玻璃試管或石英玻璃燒杯；7．實(shí)驗(yàn)樣本個數(shù)：試管型：10-30ml，可同時做20個平行反應(yīng)；燒杯型：50-250ml，可同時做4個平行反應(yīng)；主要特點(diǎn)：XH-400UV紫外光催化平行合成儀是利用紫外光的光化學(xué)原理，使反應(yīng)物分子吸收光子激發(fā)電子，分子由基態(tài)提升到激發(fā)態(tài)，從而發(fā)生光化學(xué)反應(yīng)。該儀器結(jié)合現(xiàn)代智能控制技術(shù)，在提高化學(xué)合成速度，有機(jī)物降解、工業(yè)廢水處理和環(huán)境污染等方面的研究提供了良好的方法和實(shí)驗(yàn)數(shù)據(jù)。適用于光催化有機(jī)合成、工業(yè)廢水的處理、有機(jī)物的降解、環(huán)境污染治理研究等領(lǐng)域。注：該儀器未取得中華人民共heguo醫(yī)療器械注冊證。試劑通過流路節(jié)流閥中一個很小的通道流到柱子。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津進(jìn)口基因合成儀銷售,基因合成儀" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364667226563/8161164.jpg"></div><p> 美國polyplex等。在歐洲，polygen96柱合成工作站及384柱合成塔也是較常見的工業(yè)型合成儀之一。3，大規(guī)模合成型大規(guī)模合成型主要指單柱合成產(chǎn)物量可達(dá)數(shù)克，甚至數(shù)公斤的合成儀，主要用于原料藥制備等科研或商業(yè)用途。能夠提供大規(guī)模合成儀的廠家主要為GEhealth及國產(chǎn)的pilot500。二、按試劑堿基添加方式分類，DNA合成儀可分為電磁閥氣動驅(qū)動，蠕動泵驅(qū)動兩種類型。1，電磁閥氣動驅(qū)動型：采用高于大氣壓的惰性氣體（氬氣，氮?dú)饣蚝猓閴A基試劑溶液的驅(qū)動方式，通過微量電磁閥的開合添加試劑或堿基溶液。主要品牌包括ABI系列合成儀，oligomaker系列合成儀，biolytic系列合成儀，GE系列合成儀及mermade系列DNA合成儀等。2，采用蠕動泵驅(qū)動型：采用精密蠕動泵為堿基試劑溶液的驅(qū)動方式，通過蠕動泵驅(qū)動，滑塊的相互滑動選擇添加不同堿基試劑溶液。主要品牌包括德國POLYGEN系列DNA合成儀。三、按產(chǎn)物承載容器分，可分為需要一次性合成柱，無需一次性合成儀兩類。除德國polygen系列合成儀外，其它所有品牌合成儀，如，oligomaker，mermade，ASM,POLYPLEX,ABI等都使用一次性合成柱作為合成產(chǎn)物的承載容器。入口和出口都是母路厄氏(1uer)接頭，與儀器的公路厄氏接頭配對。天津進(jìn)口基因合成儀銷售</p><p style="text-indent:25px">每一個柱子都用顏色編號，表示不同的起始核苷酸，以及有一個連續(xù)順序號碼。天津進(jìn)口基因合成儀銷售</p><p> 以亞磷酰胺寡核苷酸合成為例介紹該類儀器的一般操作步驟。亞磷酰胺DNA合成的試劑有：保護(hù)堿基的5/—DMT，A、G、C、T亞磷酰胺單體，四唑偶聯(lián)催化劑，乙酐，N—甲基咪唑封閉試劑，三氯乙酸(TCA)脫保護(hù)溶液，12氧化混合物，乙腈清洗溶劑，氨水切除溶液。使用步驟如下：1)仔細(xì)檢查合成儀上所有試劑瓶中的試劑量，必要時換掉試劑瓶，特別注意乙腈的量，因?yàn)樵撊軇┯昧枯^大。2)將標(biāo)記好的清潔的收集瓶裝在合成儀上。3)將要合成的DNA序列輸入合成儀。4)檢查選擇的合成步驟是否正確。5)選擇結(jié)束方法：TritylOffAuto是儀器的原始狀態(tài)，若打算以5，—DMT基團(tuán)連接純化寡核苷酸，將其轉(zhuǎn)變?yōu)門ritylOnAuto結(jié)束狀態(tài)。6)選擇結(jié)束方法，一般為系統(tǒng)的原始狀態(tài)。7)在每個柱監(jiān)視器的Use下，輸入你所希望的保存名字。8)以代碼代替相應(yīng)的DNA序列標(biāo)記每一個收集瓶。9)打印出每一個柱設(shè)置的詳細(xì)資料。10)檢查打印出來的資料是否正確。11)運(yùn)行StartColumn步驟檢查每一個柱的位置，確保合成儀上合成柱處于正確的位置。12)檢查連接在合成儀上的溶劑殘留(廢液瓶)是否充滿。13)如果分部收集器用來收集每個DMT脫保護(hù)步驟的流出物，確保試管充分清潔干凈，并打開分部收集器，確保DMT廢液管路通向分部收集器。天津進(jìn)口基因合成儀銷售</p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/2253017.html</p></div></div></div></div></div><div id="3qfw3k7" class="page-right twoColRt"><div id="hw39ce8" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="a8fc22l" class="recomPic likePro"><a 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