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class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364665322266/5015770.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="x3rdfrd" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364665322266/5015770.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="m3wyvxu" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>江蘇省</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>浙江直銷RNA合成儀品牌企業(yè)</p><p><span>***更新：</span>2020-06-05 12:15:26</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="imo32xe" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">昆山伯利克精密儀器有限公司</a></p><p><span>聯(lián)系人：</span>唐經(jīng)理</p><p><span>郵箱: </span>sales@biolytic.cn</p><p><span>電話: </span>18051907886</p><p><span>傳真: </span>0512_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>0512-55270018</p><p><span>地址: </span>玉楊路1038號(hào)1號(hào)樓201室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="oxztvxo" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="aphjgi3" class="box"><p><p> **初是sanger法，后來(lái)發(fā)展了幾種高通量測(cè)序方法1sanger法：雙脫氧測(cè)序的原理，DNA合成時(shí)加上一個(gè)ddNTP從而終止這條鏈的延伸，毛細(xì)管電泳讀取分析序列。一般教材講的都是這種方法，經(jīng)典，讀長(zhǎng)800bp，但是通量小成本高。2454：也稱焦磷酸測(cè)序，一個(gè)run下來(lái)約400M的數(shù)據(jù)量。將emulsionPCR產(chǎn)物連磁珠上，放入PicoTiterPlate，測(cè)序反應(yīng)是通過(guò)釋放PPi經(jīng)過(guò)ATP***化酶和熒光素酶作用發(fā)光D捕捉拍照，反應(yīng)是連續(xù)的，所以遇到連續(xù)的堿基如polyA時(shí)，454易產(chǎn)生誤差。3Solexa：邊合成邊測(cè)序，將DNA單鏈固定在芯片上，合成DNA簇，是一種可逆阻斷的合成反應(yīng)，浙江直銷RNA合成儀品牌企業(yè)，浙江直銷RNA合成儀品牌企業(yè)，每個(gè)循環(huán)只加上一個(gè)堿基、系統(tǒng)拍照一次。通過(guò)讀取拍照信號(hào)分析序列，一個(gè)run約200個(gè)G數(shù)據(jù)量以上，浙江直銷RNA合成儀品牌企業(yè)。目前能量比較高，不足是讀長(zhǎng)短，現(xiàn)在有PE150，可以測(cè)通300bp。4Soild：與454有相似之處，該方法更精細(xì)，磁珠富集，磁珠更小，密度更高。它的獨(dú)特之處是連接反應(yīng)測(cè)序，加入8堿基熒光單鏈混合物。一次測(cè)序讀兩個(gè)堿基，獲得顏色編碼組成的堿基序列，通過(guò)幾輪反應(yīng)**終得出序列。5Iontorrent：特點(diǎn)是小巧。試劑通過(guò)集成的流體通路進(jìn)入芯片中，密布于芯片上的反應(yīng)孔立即成為上百萬(wàn)個(gè)微反應(yīng)體系。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江直銷RNA合成儀品牌企業(yè)" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364665322266/5015770.jpg"></div><p> 13)如果分部收集器用來(lái)收集每個(gè)DMT脫保護(hù)步驟的流出物，確保試管充分清潔干凈，并打開(kāi)分部收集器，確保DMT廢液管路通向分部收集器。14)啟動(dòng)循環(huán)，運(yùn)行開(kāi)始程序清洗試劑管路，除去原來(lái)的試劑。注意：TritylOnAuto表示在合成寡核苷酸的5’端核苷酸帶有一個(gè)DMT，并將其從固相載體上切下來(lái)，儲(chǔ)存在收集瓶中；TritylOffAuto表示在合成寡核苷酸的5，端核苷酸沒(méi)有保護(hù)基團(tuán)，將其從固相載體上切下來(lái)，儲(chǔ)存在收集瓶中；TritylOnManual表示合成寡核苷酸的5’端核苷酸帶有一個(gè)DMT，寡核苷酸沒(méi)有從固相載體上切下來(lái)，而是保留在合成柱中；TritylOffManual表示在合成寡核苷酸的5，端核苷酸沒(méi)有保護(hù)基團(tuán)，寡核苷酸沒(méi)有從固相載體上切下來(lái)，而是保留在合成柱中。DNA合成儀日常維護(hù)編輯1．瓶塞“O”形環(huán)一月檢查一次“O”形環(huán)，**少1年更換1次。將新的備用“O”形環(huán)與儀器上的相比較，如果在“O”形環(huán)上出現(xiàn)白色沉淀，用棉花蘸取乙腈，進(jìn)行清洗。更換“O”形環(huán)的步驟如下：用止血鉗夾住“O”形環(huán)，從槽中取下(或用牙簽鉤下)，注意不要損壞托住“O”形環(huán)的白色聚氟乙烯插塞(tefloninsert)；確保插塞上沒(méi)有顆粒后，用手指將新“O”形環(huán)推進(jìn)槽，進(jìn)行壓力實(shí)驗(yàn)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江直銷RNA合成儀品牌企業(yè)" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364668652344/4756341.jpg"></div><p> DNA和RNA提取方法一、DNA的提取(方法一)1.實(shí)驗(yàn)試劑(1)DNA提取液:Tris HCl(pH),NaCl,EDTA,1％SDS(2)3MNaAc(3)TE:10mmol/LTris-HClmmol/LEDTA(4)酚():氯仿:異戊醇(25:24:1)(5)氯仿:異戊醇(24:1)(6)異丙醇(7)無(wú)水乙醇(8)75%乙醇(9)RNaseA2.實(shí)驗(yàn)步驟(1)取菌絲,在液氮中迅速研磨成粉(2)加入4mL提取液,快速振蕩混勻(3)加入等體積的4mL的氯仿:異戊醇(24:1),渦旋3～5min(此處是粗提沒(méi)有加酚,可以節(jié)約成本)(4)1000rpm,4℃,5min(5)上清用氯仿:異戊醇(24:1)再抽提一次(10,000rpm,4℃離心5min)(6)取上清,加入2/3倍體積的-20℃預(yù)冷異丙醇或,混勻,靜置約30min(7)用毛細(xì)玻棒挑出絮狀沉淀,用75%乙醇反復(fù)漂洗數(shù)次,再用無(wú)水乙醇漂洗1次,吹干,重懸于500ulTE中(8)加入1ulRNaseA(10mg/mL),37℃處理1h(9)用酚():氯仿:異戊醇(25:24:1)和氯仿:異戊醇(24:1)各抽提1次(10,000rpm,4℃離心5min)(10)取上清,1/10V3MNaAc,℃沉淀30min以上(11)沉淀用75%乙醇漂洗,風(fēng)干,溶于200ulTE中,-20℃保存?zhèn)溆枚?、DNA的提取(方法二)1.菌絲2.加入3mL65℃預(yù)熱的DNA提取緩沖液,快速振蕩混勻65℃水浴30min,期間混勻2-3次3.加入1mL5MKAc,冰浴20min4.氯仿:異戊醇(24:1)抽提1次(10,000rpm,4℃離心5min)5.取上清。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江直銷RNA合成儀品牌企業(yè)" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364748408204/4771168.jpg"></div></p><br /><p>文章來(lái)源地址: http://m.cdcfah.com/cp/157206.html</p></div></div></div></div></div><div id="t2ogias" class="page-right twoColRt"><div id="cmjqsz2" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="hqnpwik" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="3g3l8xu" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/846569.html"><div><img src="https://tyunfile.71360.com/WaterMark/UploadFile/nbzxsw/637298235378037109/764794.png" alt="寧波寧海直接考A2有幾個(gè)科目真誠(chéng)推薦寧波助學(xué)商務(wù)信息咨詢供應(yīng)" class="vcenter" 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