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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>江蘇省</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià),蛋白純化系統(tǒng)</p><p><span>***更新：</span>2020-09-06 18:02:39</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="95v9r7v" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">蘇州英賽斯智能科技有限公司</a></p><p><span>聯(lián)系人：</span>李大為</p><p><span>郵箱: </span>570745634@qq.com</p><p><span>電話: </span>18606243033</p><p><span>傳真: </span>0512_63937379</p><p><span>網(wǎng)址: </span>http://www.inscinstech.com.cn</p><p><span>手機(jī): </span>0512-63937378</p><p><span>地址: </span>吳江經(jīng)濟(jì)技術(shù)開發(fā)區(qū)聯(lián)楊路南側(cè)、龍橋路西側(cè)</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="bpxdtrh" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="zdlrhnd" class="box"><p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4 樣品的進(jìn)一步分離純化用等電點(diǎn)沉淀法、鹽析法所得到的蛋白質(zhì)一般含有其他蛋白質(zhì)雜質(zhì)，須進(jìn)一步分離提純才能得到有一定純度的樣品。常用的純化方法有：凝膠過濾層析、離子交換纖維素層析、親和層析等等。有時(shí)還需要這幾種方法聯(lián)合使用才能得到較高純度的蛋白質(zhì)樣品。 1. 凝膠過濾層析凝膠過濾層析（gel-filtration chromatography）又稱為分子排阻層析或分子篩層析。它是將葡聚糖凝膠（Sephadex）裝入一個(gè)柱子中，制成凝膠柱。這種凝膠顆粒具有網(wǎng)狀結(jié)構(gòu)，不同類型凝膠的網(wǎng)孔大小不同。當(dāng)把蛋白質(zhì)混合樣品加到凝膠柱中時(shí)，比凝膠網(wǎng)孔小的蛋白質(zhì)可進(jìn)入網(wǎng)孔內(nèi)，而大于網(wǎng)孔的分子則不能進(jìn)入被排阻在凝膠顆粒之外。當(dāng)用洗脫液洗脫時(shí)，被排阻的分子量大的蛋白質(zhì)直接通過凝膠之間的縫隙先被洗脫下來，山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià)，而比網(wǎng)孔小的蛋白質(zhì)可連續(xù)不斷地進(jìn)入網(wǎng)孔內(nèi)。這樣的小分子不但流經(jīng)的路程長，山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià)，而且受到來自凝膠內(nèi)部的阻力也很大，所以蛋白質(zhì)越小，從柱子上洗脫下來所需時(shí)間越長。由于不同蛋白質(zhì)的分子大小不同，山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià)，進(jìn)入網(wǎng)孔的程度不同，因此流出的速度不同，洗脫所用體積及時(shí)間不同，從而達(dá)到分離的目的。山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià),蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138172377738528/6371381723777385289828518.jpg"></div><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4.2 纖維素柱層析法該法是利用蛋白質(zhì)的酸堿性質(zhì)作為分離的基礎(chǔ)。離子交換纖維素（cellulose ion-exchanger）是人工合成的纖維素衍生物，它具有松散的親水性網(wǎng)狀結(jié)構(gòu)，有較大的表面積，使蛋白質(zhì)大分子可以自由通過。因此常用于蛋白質(zhì)的分離。（1）羧甲基纖維素（CM-纖維素）在纖維素顆粒上帶有羧甲基基團(tuán)。在中性pH條件下，羧甲基上的質(zhì)子可解離下來（圖2-27a），而溶液中帶正電荷的蛋白質(zhì)分子可與纖維素顆粒上的羧甲基負(fù)電荷結(jié)合?？山粨Q的基團(tuán)帶正電，因此是一種陽離子交換劑。蛋白質(zhì)與離子交換纖維素之間結(jié)合能力的大小取決于彼此間相反電荷基團(tuán)之間靜電吸引。 (2）二乙氨基乙基纖維素（DEAE-纖維素）在中性pH條件下，它含有帶正電荷的基團(tuán)，可與溶液中的帶負(fù)電荷的蛋白質(zhì)結(jié)合，可交換的基團(tuán)帶負(fù)電荷，因此是一種陰離子交換劑。當(dāng)某一蛋白質(zhì)混合溶液通過裝有DEAE-纖維素的層析柱時(shí)，帶正電荷的蛋白質(zhì)不能結(jié)合而隨著洗脫液的流動(dòng)先被洗脫下來。帶負(fù)電荷的蛋白質(zhì)將被結(jié)合到柱上。結(jié)合力取決于彼此相反電荷基團(tuán)間的靜電吸引。然后選用一定pH和離子強(qiáng)度的緩沖液進(jìn)行洗脫，改變蛋白質(zhì)分子所帶的靜電荷，依次從層析柱流出達(dá)到相互分離的目的。浙江化學(xué)分析蛋白純化系統(tǒng)制造廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià),蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171759963463/6371381717599634633342360.jpg"></div><p style="text-indent:25px">三、蛋白質(zhì)分子量的測(cè)定 3.3 沉降法沉降法又稱超速離心法。蛋白質(zhì)溶液在受到強(qiáng)大的離心力作用時(shí)，蛋白質(zhì)分子趨于下沉，沉降速度與蛋白質(zhì)分子的大小、密度和分子形狀有關(guān)，也與溶劑的密度和粘度有關(guān)。蛋白質(zhì)顆粒在離心場(chǎng)中的沉降速度用每單位時(shí)間內(nèi)顆粒下沉的距離來表示。在離心場(chǎng)中，蛋白質(zhì)分子所受到的凈離心力（離心力減去浮力）與溶劑的摩擦力平衡時(shí)，每單位離心場(chǎng)強(qiáng)度的沉降速度稱為沉降系數(shù)（Sedimentation Coefficient）。國際上采用Svedberg單位作為沉降系數(shù)的單位，用S表示，以紀(jì)念超速離心法的創(chuàng)始人，瑞典***的蛋白質(zhì)化學(xué)家T.Svedberg。一個(gè)Svedberg單位（或直接稱一個(gè)S）為1×10—13s。蛋白質(zhì)的沉降系數(shù)大約在1×10—13～200×10—13s范圍內(nèi)，即1S～200S。</p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4.3. 親和層析親和層析（affinity-chromatography）分離技術(shù)是根據(jù)許多蛋白質(zhì)對(duì)特定的化學(xué)基團(tuán)具有專一性結(jié)合的原理。這些能被生物大分子如蛋白質(zhì)所識(shí)別并與之結(jié)合的基團(tuán)稱為配基或配體（ligand）。親和層析是一種極有效的分離純化蛋白質(zhì)的方法。例如酶對(duì)它的底物具有特殊的親和力；抗原和抗體互為配基。以伴刀豆球蛋白A（concanavalin A）的分離純化為例，由于該蛋白對(duì)葡萄糖有專一性親和吸附，因此可把葡萄糖通過適當(dāng)?shù)幕瘜W(xué)反應(yīng)共價(jià)地連接到像瓊脂糖凝膠一類的載體表面上。為了防止載體表面的空間位阻影響待分離的蛋白質(zhì)大分子與其配基的結(jié)合，在配基和載體之間往往插入一段所謂的連接臂（或稱為間隔臂，spacerarm），使配體與載體之間保持足夠的距離。將這種多糖顆粒裝入一定規(guī)格的玻璃管中就制成了一根親和層析柱。當(dāng)含有伴刀豆球蛋白的提取液加到層析柱的上部，并沿柱從上往下過時(shí)，待純化的蛋白質(zhì)與其特異性配基結(jié)合而被吸附到柱上，其他蛋白因不能與葡萄糖配基結(jié)合將通過柱子而流出（圖2-28a）。然后采用一定的洗脫條件，如濃的葡萄糖溶液進(jìn)行洗脫，即可把該蛋白質(zhì)洗脫下來，達(dá)到與其它蛋白質(zhì)分離的目的。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià),蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171877557587/6371381718775575877865772.jpg"></div><p style="text-indent:25px">三、蛋白質(zhì)分子量的測(cè)定 3.2 SDS-聚丙烯酰胺凝膠電泳法測(cè)定分子量蛋白質(zhì)在普通聚丙烯酰胺凝膠中的電泳速度取決于蛋白質(zhì)分子大小、所帶電荷的量以及分子形狀。而SDS-聚丙烯酰胺凝膠電泳與此不同的是在樣品及電泳緩沖液中加入了十二烷基***鈉（sodium dodecylsulfate,SDS）。SDS是一種陰離子去污劑，可使蛋白質(zhì)變性并解離成亞基。當(dāng)?shù)鞍踪|(zhì)樣品中加入SDS（一般加入量為0.1％）后，SDS與蛋白質(zhì)分子結(jié)合，使蛋白質(zhì)分子帶上大量的負(fù)電荷，這些電荷量遠(yuǎn)遠(yuǎn)超過蛋白質(zhì)分子原來所帶的電荷量，因而掩蓋了不同蛋白質(zhì)之間的電荷差異。所有結(jié)合SDS的蛋白質(zhì)-SDS復(fù)合物的形狀近似于長的橢園棒，它們的短軸是恒定的，而長軸與蛋白質(zhì)分子量的大小成正比。這樣，消除了蛋白質(zhì)之間原有的電荷和形狀的差異，電泳的速度只取決于蛋白質(zhì)分子量的大小。進(jìn)行凝膠電泳時(shí)，常常用一種染料作前沿物質(zhì)，蛋白質(zhì)分子在電泳中的移動(dòng)距離和前沿物質(zhì)移動(dòng)的距離之比值稱為相對(duì)遷移率，相對(duì)遷移率和分子質(zhì)量的對(duì)數(shù)成直線關(guān)系。以標(biāo)準(zhǔn)蛋白質(zhì)分子質(zhì)量的對(duì)數(shù)和其相對(duì)遷移率作圖，得到標(biāo)準(zhǔn)曲線。將未知蛋白質(zhì)在同樣條件下電泳，根據(jù)測(cè)得的樣品相對(duì)遷移率，從標(biāo)準(zhǔn)曲線上便可查出其分子量。山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià)</p><p style="text-indent:25px">山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià)</p><p style="text-indent:25px">儀器儀表行業(yè)本身是一個(gè)加入，產(chǎn)出周期較長的行業(yè)，所以作為業(yè)內(nèi)廠家，一方面要尋找中、短期市場(chǎng)熱點(diǎn)，另一方面也要在產(chǎn)品、技術(shù)研發(fā)方面有長遠(yuǎn)的規(guī)劃;中國的新型工業(yè)化進(jìn)程，信息化和工業(yè)化融合的進(jìn)一步加深，帶動(dòng)各個(gè)工業(yè)領(lǐng)域?qū)τ诘鞍准兓V系統(tǒng)，凝膠凈化色譜系統(tǒng)，制備液相色譜，柱塞泵等產(chǎn)品的需求。目前我國機(jī)器人與自動(dòng)化設(shè)備、機(jī)械電子設(shè)備的研發(fā)、生產(chǎn)、銷售及技術(shù)咨詢；光學(xué)檢測(cè)設(shè)備、光電產(chǎn)品研發(fā)、制造、銷售；實(shí)驗(yàn)室設(shè)備、儀器儀表的研發(fā)、制造、銷售及提供相關(guān)的技術(shù)咨詢和售后服務(wù)；自營和代理各類商品及技術(shù)的進(jìn)出口業(yè)務(wù)（限定企業(yè)經(jīng)營或禁止進(jìn)出口的商品和技術(shù)除外）。（依法需經(jīng)批準(zhǔn)的項(xiàng)目，經(jīng)相關(guān)部門批準(zhǔn)后方可開展經(jīng)營活動(dòng)）產(chǎn)品，主要集中在中低檔市場(chǎng)，而市場(chǎng)則主要被國外品牌所占據(jù)。在某些領(lǐng)域，國產(chǎn)產(chǎn)品甚至是空白，這就需要未來我國儀器儀表向市場(chǎng)進(jìn)軍，擴(kuò)大產(chǎn)品占比。從加工廣義角度來說，儀器儀表也可具有自動(dòng)操控、報(bào)警、信號(hào)傳遞和數(shù)據(jù)處理等功能，例如用于工業(yè)生產(chǎn)過程自動(dòng)操控中的氣動(dòng)調(diào)節(jié)儀表，和電動(dòng)調(diào)節(jié)儀表，以及集散型儀表操控系統(tǒng)也皆屬于儀器儀表。山東直銷蛋白純化系統(tǒng)廠家報(bào)價(jià)</p><p style="text-indent:25px">蘇州英賽斯智能科技有限公司致力于儀器儀表，以科技創(chuàng)新實(shí)現(xiàn)***管理的追求。英賽斯擁有一支經(jīng)驗(yàn)豐富、技術(shù)創(chuàng)新的專業(yè)研發(fā)團(tuán)隊(duì)，以高度的專注和執(zhí)著為客戶提供蛋白純化色譜系統(tǒng)，凝膠凈化色譜系統(tǒng)，制備液相色譜，柱塞泵。英賽斯繼續(xù)堅(jiān)定不移地走高質(zhì)量發(fā)展道路，既要實(shí)現(xiàn)基本面穩(wěn)定增長，又要聚焦關(guān)鍵領(lǐng)域，實(shí)現(xiàn)轉(zhuǎn)型再突破。英賽斯始終關(guān)注自身，在風(fēng)云變化的時(shí)代，對(duì)自身的建設(shè)毫不懈怠，高度的專注與執(zhí)著使英賽斯在行業(yè)的從容而自信。</p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/1182695.html</p></div></div></div></div></div><div id="p7dbjp7" class="page-right twoColRt"><div id="xbhfflb" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="tj7jv97" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 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