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href="http://m.cdcfah.com/cp/second-yiqiyibiao_zyyqyb/">**儀器儀表</a>?<a href="http://m.cdcfah.com/cp/third-zyyqyb_qtzyyqyb/">其他**儀器儀表</a>?廣東新品基因合成儀哪里有值得信賴昆山伯利克精密儀器供應(yīng)</p><div id="tnp8vsp" class="leftbox clearfix"><h1>廣東新品基因合成儀哪里有值得信賴昆山伯利克精密儀器供應(yīng)</h1><div id="bk72q2v" class="proBig"><div id="dcod3o7" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364679628907/6766793.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="a8zovkg" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364679628907/6766793.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="veqnk7t" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>江蘇省</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>廣東新品基因合成儀哪里有,基因合成儀</p><p><span>***更新：</span>2020-09-01 19:18:33</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="733eglm" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">昆山伯利克精密儀器有限公司</a></p><p><span>聯(lián)系人：</span>唐經(jīng)理</p><p><span>郵箱: </span>sales@biolytic.cn</p><p><span>電話: </span>18051907886</p><p><span>傳真: </span>0512_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>0512-55270018</p><p><span>地址: </span>玉楊路1038號1號樓201室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="0ugs8o4" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="udaxul8" class="box"><p><p> 以亞磷酰胺寡核苷酸合成為例介紹該類儀器的一般操作步驟。亞磷酰胺DNA合成的試劑有：保護(hù)堿基的5/—DMT，A、G、C，廣東新品基因合成儀哪里有、T亞磷酰胺單體，四唑偶聯(lián)催化劑，乙酐，N—甲基咪唑封閉試劑，廣東新品基因合成儀哪里有，三氯乙酸(TCA)脫保護(hù)溶液，12氧化混合物，乙腈清洗溶劑，氨水切除溶液。使用步驟如下：1)仔細(xì)檢查合成儀上所有試劑瓶中的試劑量，必要時換掉試劑瓶，特別注意乙腈的量，因為該溶劑用量較大。2)將標(biāo)記好的清潔的收集瓶裝在合成儀上。3)將要合成的DNA序列輸入合成儀。4)檢查選擇的合成步驟是否正確。5)選擇結(jié)束方法：TritylOffAuto是儀器的原始狀態(tài)，若打算以5，—DMT基團(tuán)連接純化寡核苷酸，將其轉(zhuǎn)變?yōu)門ritylOnAuto結(jié)束狀態(tài)。6)選擇結(jié)束方法，一般為系統(tǒng)的原始狀態(tài)。7)在每個柱監(jiān)視器的Use下，輸入你所希望的保存名字。8)以代碼代替相應(yīng)的DNA序列標(biāo)記每一個收集瓶。9)打印出每一個柱設(shè)置的詳細(xì)資料。10)檢查打印出來的資料是否正確。11)運行StartColumn步驟檢查每一個柱的位置，確保合成儀上合成柱處于正確的位置。12)檢查連接在合成儀上的溶劑殘留(廢液瓶)是否充滿。13)如果分部收集器用來收集每個DMT脫保護(hù)步驟的流出物，確保試管充分清潔干凈，并打開分部收集器，確保DMT廢液管路通向分部收集器。細(xì)檢查合成儀上所有試劑瓶中的試劑量，必要時換掉試劑瓶，廣東新品基因合成儀哪里有，特別注意乙腈的量，因為該溶劑用量較大。廣東新品基因合成儀哪里有</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="廣東新品基因合成儀哪里有,基因合成儀" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364679628907/6766793.jpg"></div><p> DNA合成儀一般需堿基瓶組及試劑瓶組。試劑瓶組一般**少為6個，含脫保護(hù)劑（Deb）、活化劑（act）、蓋帽試劑A（capA)、蓋帽試劑B(capB)、氧化劑（OXI）及洗脫乙腈（ACN），每種儀器一般都多設(shè)置1-2個試劑瓶位，用于特殊產(chǎn)物的合成。堿基瓶組一般**少為4個標(biāo)準(zhǔn)堿基（A/C/G/T）,同時搭配2-多個特殊堿基瓶位，用于熒光標(biāo)記等合成。 </p> <p> DNA 合成儀的一般原則需要保持內(nèi)外氣體隔絕，因此無論試劑瓶、堿基瓶均需密封保持一定壓力。所有壓力管路均采用特氟龍導(dǎo)管，直徑為1/16—1/8吋。每個瓶子都有一個氬氣壓力管道及輸送管道進(jìn)入瓶蓋插塞，對于亞磷酰胺，1—8號瓶其壓力管道也作為排氣管。氬氣管要保持在液體水平以上，而輸送管卻伸到瓶的底部。當(dāng)閥門正確設(shè)置打開時，儲液瓶上部空間由氬氣加壓，液體被推進(jìn)輸送管，流到其目的地。廣東新品基因合成儀哪里有試劑通過底部的luer接頭流到柱子，如果沒擰上下面的一個luer接頭應(yīng)會在一個圓柱形的玻璃流路節(jié)流閥上發(fā)現(xiàn)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="廣東新品基因合成儀哪里有,基因合成儀" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364668056641/5786720.jpg"></div><p> 二、按試劑堿基添加方式分類，DNA合成儀可分為電磁閥氣動驅(qū)動，蠕動泵驅(qū)動兩種類型。1，電磁閥氣動驅(qū)動型：采用高于大氣壓的惰性氣體（氬氣，氮氣或氦氣）為堿基試劑溶液的驅(qū)動方式，通過微量電磁閥的開合添加試劑或堿基溶液。主要品牌包括ABI系列合成儀，oligomaker系列合成儀，biolytic系列合成儀，GE系列合成儀及mermade系列DNA合成儀等。2，采用蠕動泵驅(qū)動型：采用精密蠕動泵為堿基試劑溶液的驅(qū)動方式，通過蠕動泵驅(qū)動，滑塊的相互滑動選擇添加不同堿基試劑溶液。主要品牌包括德國POLYGEN系列DNA合成儀。三、按產(chǎn)物承載容器分，可分為需要一次性合成柱，無需一次性合成儀兩類。除德國polygen系列合成儀外，其它所有品牌合成儀，如，oligomaker，mermade，ASM,POLYPLEX,ABI等都使用一次性合成柱作為合成產(chǎn)物的承載容器。 </p><p> 發(fā)展：編輯當(dāng)前DNA合成儀的主要生產(chǎn)廠商都致力于機(jī)器性能的完善和應(yīng)用領(lǐng)域的開拓以及高效率、高產(chǎn)率的儀器的開發(fā)與研究。中國臺灣科學(xué)委yuan會“基因醫(yī)藥衛(wèi)生科技尖duan研究計劃”中的基因生物技術(shù)組已經(jīng)成功研發(fā)全世界第yi臺高密度復(fù)制DNA合成儀，可以同時合成384條不同DNA，每日可合成768條DNA，并可以配合聚合酶連鎖反應(yīng)裝置，大量復(fù)制各種基因，不但節(jié)省時間，也可節(jié)省大量人力，這部機(jī)器能復(fù)制動物、人體、植物的基因甚至是防偽基因。由于人們所需要的大部分核酸的堿基對數(shù)量遠(yuǎn)遠(yuǎn)超過DNA合成儀可以合成的**長核酸鏈的堿基對數(shù)量，因此還需要不斷改善合成工藝才能得到較長的核酸分子。為了能夠在將來改變酶的結(jié)構(gòu)，使其在工業(yè)上更有價值；改變蛋白質(zhì)和多肽的結(jié)構(gòu)，制備新藥wu；并開拓有關(guān)人類疾病和遺傳調(diào)控的新領(lǐng)域，化學(xué)合成DNA在這一過程中將起關(guān)鍵性作用。根據(jù)不同化學(xué)方法研制的DNA合成儀也將不斷涌現(xiàn)，能夠突破現(xiàn)有核酸合成的堿基對數(shù)量限制，大量節(jié)省人力、物力、財力。每一個5—端口柱閥塊將流出物導(dǎo)入廢液口、DMT收集口，或DNA收集瓶。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="廣東新品基因合成儀哪里有,基因合成儀" src="http://tyunfile.71360.com/UploadFile/ksblkjmyq/637200364748408204/4771168.jpg"></div><p style="text-indent:25px">一、按照不同用途，DNA合成儀可分為實驗室型，工業(yè)型和大規(guī)模合成三種主要類型。1，實驗室型：主要指合成通量較小，且單柱合成產(chǎn)物為10-1000nmol的DNA合成儀，一般為合成柱數(shù)低于48柱（含）的DNA合成儀，主要適用于化學(xué)生物學(xué)實驗室，或某些剛起步的商業(yè)機(jī)構(gòu)。該類型DNA合成儀品牌較多，包括已停產(chǎn)的ABI391,394,3400,3900，BECKMAN oligo-1000，及仍然量產(chǎn)的polygen 10柱，12柱，mermade4，6,8,12,48柱；BIOSSET ASM-800， AZCO OLIGO-8等。軟件儲存在一個可取出的存儲卡中，這個存儲卡插在儀器的背面。廣東新品基因合成儀哪里有</p><p style="text-indent:25px">如果分部收集器用來收集每個DMT脫保護(hù)步驟的流出物。廣東新品基因合成儀哪里有</p><p> PSI200多通道多肽合成儀1.技術(shù)參數(shù)通道數(shù)3或6通道反應(yīng)器10ml、20ml或40ml合成規(guī)模試劑儲罐4個500ml溶劑儲罐（標(biāo)準(zhǔn)配置）1個30L、1個2L、2個500ml試劑儲罐（與PSI400D匹配）反應(yīng)器攪拌模式180度或270度上下翻轉(zhuǎn)的無死角機(jī)械攪拌模式反應(yīng)器攪拌速度無極可調(diào)反應(yīng)器攪拌強(qiáng)度無極可調(diào)合成樹脂攪拌不傷樹脂合成過程全程氮氣保護(hù)合成方式Fmoc/tBoc活化方式預(yù)活化、沉淀活化和非沉淀活化合成步驟4至6步即可完成氨基酸的合成耦合效率耦合效率可達(dá)99%以上主要特點：1.耦合效率高?0度-180度機(jī)械上下轉(zhuǎn)動混合，反應(yīng)徹底無死角，樹脂無損壞且完全參加反應(yīng)，平均氨基酸的耦合率都在99％以上；?與氮氣鼓泡式、頂置式、搖床、鐘擺式、渦旋式等攪拌方式相比180度上下翻轉(zhuǎn)效果zui佳;?很多多肽科學(xué)家手工試驗證明：180度機(jī)械攪拌方式是多肽合成的zui佳方式。2.零耗材設(shè)計?運行費用幾乎為零；?儀器內(nèi)部管路和反應(yīng)器都采用了316L不銹鋼過濾膜，永jiu不需更換；?試劑儲罐采用實驗室通用器材，客戶可方便且便宜地買到；3.程序簡單完美，是多肽合成儀中**為優(yōu)化的一種?指令程序編制模塊化人性化，可以方便地對每一參數(shù)進(jìn)行調(diào)整，甚至重新編排，方便使用者學(xué)習(xí)、編輯、修改和運行。廣東新品基因合成儀哪里有</p><p style="text-indent:25px">昆山伯利克精密儀器有限公司致力于儀器儀表，是一家生產(chǎn)型的公司。公司業(yè)務(wù)分為DNA/RNA引物合成儀，768道合成儀，192c合成儀，48合成儀等，目前不斷進(jìn)行創(chuàng)新和服務(wù)改進(jìn)，為客戶提供良好的產(chǎn)品和服務(wù)。公司從事儀器儀表多年，有著創(chuàng)新的設(shè)計、強(qiáng)大的技術(shù)，還有一批**的專業(yè)化的隊伍，確保為客戶提供良好的產(chǎn)品及服務(wù)。昆山伯利克秉承“客戶為尊、服務(wù)為榮、創(chuàng)意為先、技術(shù)為實”的經(jīng)營理念，全力打造公司的重點競爭力。</p></p><br /><p>文章來源地址: http://m.cdcfah.com/cp/1121561.html</p></div></div></div></div></div><div id="ts8hj8p" class="page-right twoColRt"><div id="hwyahjq" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="ixzgxe2" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="n8z4xpm" class="recomPic 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