江蘇9分鐘完成RNA提取試劑盒500T/5865元 **** 上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買(mǎi)產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content=" RNA常用提取方法（1）1、異硫氰酸胍氯化銫超速離心法: 原理：使用蛋白質(zhì)變性劑異硫氰酸胍有效制抑RNA酶的活性，經(jīng)過(guò)起始密度為1.78g/ml的氯化銫介質(zhì)，進(jìn)行密度梯度超速離心，RNA沉淀于管"><meta name="keywords" content="江蘇9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒"><link rel="canonical" href="http://m.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" 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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒</p><p><span>***更新：</span>2020-08-27 03:29:50</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="73hj26w" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="wcrgkts" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="ijdrk8f" class="box"><p><p> <br /> </p> <p> </p> <p> <br /> </p> <p> RNA 常用提取方法（1） </p> <p> 1、異硫氰酸胍氯化銫超速離心法: </p> <p> 原理：使用蛋白質(zhì)變性劑異硫氰酸胍有效制抑RN A 酶的活性，經(jīng)過(guò)起始密度為1.78g/m l 的氯化銫介質(zhì)，進(jìn)行密度梯度超速離心，RNA沉淀于管底，而DNA與蛋白質(zhì)在上清中。使用這種方法，不但能得到高質(zhì)量的RNA ，而且能同時(shí)分離出細(xì)胞染色體DNA。 </p> <p> </p> <p> 2、鹽酸胍-有機(jī)溶劑法： </p> <p> 原理：本法有MacDonald1987年在Strohman報(bào)道的方法基礎(chǔ)上改進(jìn)而成的，適用于沒(méi)有超速離心及設(shè)備的情況下提取細(xì)胞總RNA。它利用鹽酸胍制抑RNA酶，勻漿裂解細(xì)胞，有機(jī)溶劑抽提去除蛋白質(zhì),通過(guò)選擇性沉淀RNA分子而去除DNA。 </p> <p> </p> <p> 3，江蘇9分鐘完成RNA提取試劑盒500T/5865元、氯化鋰-尿素法： </p> <p> 原理：本法首先由Auffray報(bào)道,利用高濃度尿素變性蛋白質(zhì)同時(shí)制抑RNA酶，3 mol/L LiCl選擇性沉淀RNA，江蘇9分鐘完成RNA提取試劑盒500T/5865元，江蘇9分鐘完成RNA提取試劑盒500T/5865元。 </p> <p> </p> <p> <br /> </p> <p> <br /> 凡知醫(yī)學(xué)基于自主創(chuàng)新的試劑凍干技術(shù)、等溫?cái)U(kuò)增技術(shù)、微流控芯片技術(shù)等開(kāi)發(fā)了一體化基因快速檢測(cè)儀。江蘇9分鐘完成RNA提取試劑盒500T/5865元</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414531250/8063352.png"></div><p> RNA提取注意事項(xiàng)（1）: </p> <p> 迅速滅活內(nèi)源的RNA酶，以防止RNA降解。以下3個(gè)方法均可有效使內(nèi)源RNA酶失活： 1）用含離液（如胍鹽）的細(xì)胞裂解液收獲樣品，并立即勻漿。 2）用液氮瞬間凍結(jié)樣品。值得特別注意的是：組織塊必須保證足夠小，在浸入液氮的瞬間就能凍結(jié)，以確保瞬間令RNA酶失活。3）立即將樣品置于RNAfixer無(wú)液氮RNA樣品儲(chǔ)存液中。它是一種水相、的收集試劑，能立即穩(wěn)定并保護(hù)完整、未凍結(jié)的組織和細(xì)胞樣品中的RNA。關(guān)鍵要點(diǎn)是組織樣品切片一定要夠薄（<0.5 cm），這樣RNAfixer才能在RNase破壞RNA之前迅速滲入組織塊中。江蘇9分鐘完成RNA提取試劑盒500T/5865元將吸附柱放入新管中，向膜中央滴加50-100ul RNase free ddH2O。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116738986650391/4176717.png"></div><p> 蛋白和多糖污染可能的原因如下： </p> <p> 樣品中蛋白和多糖含量高，建議加入氯仿之前先高速離心去除蛋白和多糖。 </p> <p> 吸取上層無(wú)色水相時(shí)不小心吸入一些中間蛋白層的物質(zhì)，建議將移液***頭中的液體放回分層的離心管中，重新進(jìn)行離心后再小心吸取上層水相即可。 </p> <p> 樣品量太大，裂解不完全也會(huì)導(dǎo)致 RNA 樣品中蛋白和多糖污染。 </p> <p> A260/A280 的比值低于 1.65 </p> <p> 可能的原因如下：1、RNA 樣品溶于 TE 溶液，低離子濃度和低 pH 條件下，A280 的數(shù)值會(huì)較高，導(dǎo)致比值低。 </p> <p> 樣品勻漿時(shí)加的 Trizol 試劑量太少。 </p> <p> 勻漿后樣品未在室溫放置 5 分鐘，會(huì)導(dǎo)致蛋白的析出不完全，也會(huì)增加 A280的數(shù)值。 </p> <p> ***得到的 RNA 沉淀溶解不完全。 </p> <p> <br /> </p><p> SimplyP 總RNA提取試劑 </p> <p> <br /> </p> <p> 把總RNA提取速度提高3倍，僅9分鐘內(nèi)完成！ </p> <p> 產(chǎn)品特點(diǎn)： </p> <p> 1.速度快：9分鐘內(nèi)可獲得RNA。 </p> <p> 2.步驟少：4步即可完成。 </p> <p> 3.操作簡(jiǎn)單：SimplyP試劑盒內(nèi)只有四種試劑，僅需經(jīng)過(guò)裂解、吸附過(guò)濾、洗滌、洗脫即可，提純整個(gè)操作過(guò)程簡(jiǎn)單易行，且提取的RNA質(zhì)量不受操作者熟練程度的影響。 </p> <p> 4.適用范圍廣：可用于組織、全血、體液、骨髓、細(xì)胞等樣品，且試劑盒中有可徹底滅活病原體的試劑，對(duì)于具有潛在傳染性的臨床樣本RNA的提取，有安全、可靠、不產(chǎn)生交叉污染的特殊優(yōu)勢(shì)。 </p> <p> 5.保質(zhì)期長(zhǎng)：由于本試劑盒采用的裂解液是中性的，沒(méi)有腐蝕性，且不易變質(zhì)，使得該試劑盒可室溫保存兩年。使用時(shí)一定要根據(jù)自己的實(shí)驗(yàn)需要購(gòu)買(mǎi)專(zhuān)用提取試劑盒，如果不是專(zhuān)用試劑盒。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414521485/5765636.png"></div><p> 可選步驟： 4℃ 12,000 rpm (~13,400×g) 離心5分鐘，取上清，轉(zhuǎn)入一個(gè)新的無(wú)RNase 的離心管中。 </p> <p> 注意：如果樣品中含有較多蛋白、脂肪、多糖或肌肉、植物結(jié)節(jié)部分等，可加此步驟離心去除。離心得到的沉淀中包括細(xì)胞外膜、多糖、高分子量DNA，RNA存在于上清溶液中。 </p> <p> 加入200 μl 氯仿，蓋好管蓋，劇烈振蕩15 s，室溫放置3 min。 </p> <p> 4℃ 12,000 rpm (~13,400×g) 離心10 min，樣品會(huì)分成三層：黃色的有機(jī)相，中間層和無(wú)色的水相，RNA 主要在水相中，水相的體積約為所用溶液 RL試劑的60%。把水相轉(zhuǎn)移到新管中，進(jìn)行下一步操作。***次使用前應(yīng)在溶液 RPI、溶液 RW中加入無(wú)水乙醇，加入量請(qǐng)參見(jiàn)瓶上標(biāo)簽。取新鮮或-70℃凍存100mg**在液氮中研磨，把粉末加入到1ml裂解液中混勻。天津操作簡(jiǎn)單RNA提取試劑盒QQ 804036498</p><p style="text-indent:25px">或許能提出RNA，但不能確保其質(zhì)量以及完整性，會(huì)影響RT-PCR。江蘇9分鐘完成RNA提取試劑盒500T/5865元</p><p> 向吸附柱中加入600ul漂洗液(使用前請(qǐng)先檢查是否已加入無(wú)水乙醇)，2-8 12,000 rpm ℃ 離心2min，棄廢液。 </p> <p> 向吸附柱中加入600ul漂洗液，2-8 12,000 rpm ℃ 離心2min，棄廢液。 </p> <p> 12000rpm離心2min，棄掉收集管，將吸附柱置于室溫放置數(shù)分鐘將吸附柱中殘余的漂洗液去除。 </p> <p> 將吸附柱放入新管中，向膜中央滴加50-100ul RNase free ddH2O，室溫放置5min，12000rpm室溫離心2min即得到RNA </p> <p> <p> 所有相關(guān)器皿耗材都應(yīng)為RNase-free產(chǎn)品，操作過(guò)程要小心，帶口罩、手套避免環(huán)境中RNA酶污染樣品。 </p> <p> <br /> </p> 江蘇9分鐘完成RNA提取試劑盒500T/5865元</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)辦于2003-01-22，是一家服務(wù)型的公司。經(jīng)過(guò)多年不斷的歷練探索和創(chuàng)新發(fā)展，公司是一家有限責(zé)任公司企業(yè)，以誠(chéng)信務(wù)實(shí)的創(chuàng)業(yè)精神、優(yōu)質(zhì)高速的管理團(tuán)隊(duì)、精悍的職工隊(duì)伍，努力為廣大用戶提供高品質(zhì)的產(chǎn)品。公司始終堅(jiān)持客戶需求優(yōu)先的原則，致力于提供高質(zhì)量的科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷(xiāo)售。上海朝瑞科技自成立以來(lái)，一直堅(jiān)持走正規(guī)化、專(zhuān)業(yè)化路線，得到了廣大客戶及社會(huì)各界的普遍認(rèn)可與大力支持。</p></p><br /><p>文章來(lái)源地址: http://m.cdcfah.com/cp/1054380.html</p></div></div></div></div></div><div id="q3bocr3" class="page-right twoColRt"><div id="gq9p6f7" class="box01 margin10 clearfix"><h3><a href="http://m.cdcfah.com/cp/">猜你喜歡</a></h3><div id="y4sg4hi" class="recomPic likePro"><a href="http://m.cdcfah.com/cp/814588.html"><div><img 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